Plasmids

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AAV-actin prom-dsRed-scramble shRNA

AAV vector to express dsRed sh RNA (scramble)

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pCK302

As pCK301, but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be used

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pMV306G13+Lux

Bioluminescent reported plasmid to express bacterial luciferase (Lux) in mycobacteria.

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pMV306hsp+LuxG13

Bioluminescent reported plasmid to express bacterial luciferase (Lux) in mycobacteria.

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pMV306hsp+FFlucWT

Bioluminescent reported plasmid to express firefly luciferase (FFluc) in mycobacteria.

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pAAV-flex-rev-g2-2A-Venus

Confers zolpidem sensitivity on zolpidem-insensitive neurons

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pCK301

E.coli rhaBAD promoter upstream of sfGFP

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pCK300

Empty control plasmid, three terminators to place inserts between for insulation, ampR, pBR322 origin, lacI

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pPRIME-dsRed-shscramble

Expresses dsRed and scramble shRNA under the control of the CMV promoter in eukaryotic cells

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pPRIME-dsRed-shvgat

Expresses dsRed and shRNA against vgat under the control of the CMV promoter in eukaryotic cells

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AAV-actin prom-dsRed-adra2a.1227.shRNA

Expresses dsRed and shRNA to knock down adra2a receptors

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phOct4-EGFP

Expresses enhanced GFP under control of OCT4

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pAAV-hysn-flex-dsRed-shscramble

Expresses scramble shRNA Cre-dependently by flex switch

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pAAV-hsyn-flex-dsRed-shvgat

Expresses shRNA to the vesicular GABA transporter VGAT. Cre dependent expression by flex switch

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pAAV-cFos-tTA-pA

Expresses TetActivator protein under the control of the c-fos promoter for use in TetTaging

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pAAV-PTRE-tight-hM3Dq-mCherry

Expresses the DREADD receptor hM3Dq-mCherry under the control of tet-inducible promoter for use in TetTaging

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pNestin-EGFP

Expression of GFP specifically in neural progenitors

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pDiGc

Inducible expression of dsRed and constitutive expression of GFP in bacteria

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pDiGi

Inducible expression of dsRed and GFP in bacteria

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pFCcGi

Inducible expression of GFP and constitutive expression of mCherry

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pCK314

Modified rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites.

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pMV306hsp

Mycobacterial integrated expression vector with strong hsp60 promoter

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pMEND-Lx

Mycobacterial Tetracycline Inducible Luciferase Vector

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pKW08-Lux

Mycobacterial Tetracycline Inducible Luciferase Vector

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pMEND-Lux-Int

Mycobacterial Tetracycline Inducible Luciferase Vector (integration version)

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pKW08-Lux-Int

Mycobacterial Tetracycline Inducible Luciferase Vector (integration version)

 

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pMV306hsp+Lux reporter plasmid

Reporter plasmid for research into tuberculosis treatments or the pathogenesis of M. tuberculosis

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pMV306hspFFLuc reporter plasmid

Reporter plasmids for research into tuberculosis treatments or the pathogenesis of M. tuberculosis

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pMV306hsp+Gluc reporter plasmid

Reporter plasmids for research into tuberculosis treatments or the pathogenesis of M. tuberculosis

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pCK306

rhaBAD, a rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites.

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BASIC_5_Amp

Storage vector for BASIC part: Amp

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BASIC_4_Cm

Storage vector for BASIC part: Cm

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BASIC_10_J23101-RBS32-GFP-B0015

Storage vector for BASIC part: GFP expression cassette

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BASIC_12_ATG-GFP-B0015

Storage vector for BASIC part: GFP(ORF)

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BASIC_13_ATG-GFP-nostop

Storage vector for BASIC part: GFP(ORF)-nostop

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BASIC_6_J23102

Storage vector for BASIC part: J23102

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BASIC_3_Kan

Storage vector for BASIC part: Kan

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BASIC_2_Kan-MB1

Storage vector for BASIC part: Kan-MB1

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BASIC_1_MB1

storage vector for BASIC part: MB1

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BASIC_11_RBS34-GFP-B0015

Storage vector for BASIC part: RBS-GFP(ORF)

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BASIC_8_RBS34- mCherry -B0015

Storage vector for BASIC part: RBS-RFP(ORF)

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BASIC_7_J23101-RBS34-mCherry-B0015

Storage vector for BASIC part: RFP expression cassette

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T17 Multi-gene Murine Cytokine/Chemokine Plasmid

T17 Multi-gene Murine Cytokine/Chemokine Plasmid for Quantitative PCR

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T18 Multigene Human Cytokine/Chemokine Plasmids

T18 Multigene Human Cytokine/Chemokine Plasmids for Quantitative PCR

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T19 Multigene Rabbit Cytokine/Chemokine Plasmids

T19 Multigene Rabbit Cytokine/Chemokine Plasmids for Quantitative PCR

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