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A portfolio of human colorectal adenocarcenoma cell lines including primary cell lines isolated from patients (of various Duke's stages and histological grades) in addition to cloned cells lines genetically modified to highly express alpha integrin domains.

Primary

Six cell lines from primary human colorectal adenocarcinomas were established from surgical specimens without the use of 'feeder' cells, 'conditioned' medium or passage of cells in nude mice. The six cell lines exhibit considerable variation in morphology, CEA secretion and tumourigenicity in nude mice. At least two of the lines retain some of the differentiated characteristics of colorectal epithelium. HRA-19 has stem cell properties and undergoes a multilineage differentiation in serum free medium. 

Clonal

HRA-19a1.1 is a clone derived from HRA-19. The HRA-19a1.1 cell line was transfected with alpha integrin domain coding plasmids to create cell lines highly expressing alpha2 or alpha2 and alpha 1 integrin. In experiments, cell lines expressing alpha2 showed a significantly increased endrocrine and mucous cell lineage commitment relative to the parent cell line.


 Data from: S C Kirkland and H Ying (2008) α2β1 Integrin regulates lineage commitment in multipotent human colorectal cancer cells, J Biol Chem, 283(41): 27612-27619

 

These cell lines are available through Public Health England ECACC. Please make your purchase through the links below.

Imperial Innovations requests that commercial/for profit parties contact them first to discuss the terms of supply prior to Innovations authorising the release of supplies from ECACC.

Please email quicktech@imperialinnovations.co.uk to discuss the release of these supplies.

Human colorectal adenocarcinomas

Portfolio of human colorectal adenocarcinomas

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