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Background: Cardiac myocytes are electrically coupled by gap junctions, clusters of intercellular channels composed of connexins. Variations in the quantities and spatial distribution of different connexin types have been implicated in regional differentiation of electrophysiological properties in the heart.  Cardiac myocytes are reported to express connexin43, connexin40, and connexin45.

Invention: Hybridoma producing antibodies against Connexin 45 (Cx45), a component of the gap junction that is present in specialised region of heart (The sino-atrial node and the Atrio-ventricular node particularly). 

Characterisation of new monoclonal antibody to Cx45 Q14E-(mab19-11-5)

Figure: Characterisation of a new monoclonal antibody to Cx45, Q14E-(mab19-11-5). (A) western blot of cells transfected with connexins 43, 40 and 45 and wild type cells probed with the new antibody. A reactive band is observed at 45 kDa only in the lane containing cells transfected with Cx45.

This reaction is inhibited when the antibody is pre-incubated with the peptide immunogen. (B) The Cx45-transfected HeLa cell line immunolabelled with the new monoclonal antibody. Punctate label is observed between the cells. Again, this labelling is inhibited by pre-incubation with the peptide immunogen (C). Labelling of the rat AV bundle by the previously characterised anti-Cx45, GP42 (D), is reproduced by the new monoclonal antibody (E). These data confirm specificity of the new monoclonal antibody. Bar markers = 50 µm (B and C) and 100 µm (D and E)

Antigen/Gene or Protein Targets: Connexin 45

Host: Mouse

Applications: Immunoflorescence and Western blot experiments

Summary & Benefits: This reagent has been shown to label the protein in immunofluorescence and western blot experiments.

Depositor: Emmanuel Dupont, National Heart & Lung Institute, Imperial College London, Hammersmith Campus, DuCane Road, London, W12 0NN and BioGenes GmbH, Köpenicker Str. 325, D-12555 Berlin

 

Internal case number: 6999

Anti connexin 45 antibodies (Q14E-(mab19-11-5))

Hybridoma cell line

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