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HMEC-1 (human microvascular endothelial cell line) expressing a synthetic gene network which produces enhanced green fluorescent protein (eGFP) in response to wall shear stress applied on the cultured cell monolayer. 

Useful for research in vascular function and disease.

 

 

The mechanosensitive synthetic gene network consists of a sensor module, a reporter module, and a linker module that signals between the sensor and reporter. The sensor module is composed of the Bradykinin B2 G-PCR linked to the tetracycline-controlled transcriptional trans-activator (tTA).The linker between the GPCR and tTA is a 7 amino acid long Tobacco etch virus (TEV) protease cleavage site. The reporter module contains eGFP under the control of a tTA dependent promoter. The linker module is βArrestin2 signalling protein fused to the TEV protease enzyme. Upon activation of the G-PCR by shear stress or by an extracellular ligand (bradykinin), βArrestin2 binds to the cytoplasmic domain of the G-PCR, causing the cleavage of the linker between tTA and the G-PCR by the TEV protease. Consequently, the released tTA promotes transcription from the reporter module.

Internal case number: 7553

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Mechanosensing Fluorescent HMEC-1 Cell Line

Human microvascular endothelial cell line in which GFP expression is stimulated by mechanical force

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