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Novel system enabling researchers to amplify and compare expression of multiple genes in a quicker and less resource-consuming way.

Setting up plasmid standard curves for quantitative RT-PCR can be time-consuming. This novel system allows researchers to amplify and compare expression of multiple genes in a quicker and less resource-consuming way. It reduces the variation between individual gene PCR amplification results due to plasmid dilutional errors. Multi-gene plasmid reduces the necessity to maintain multiple plasmid stocks and significantly reduces time and labour costs associated with RT-PCR as only one serial dilution of plasmid is required for all the genes.

Reagent:

 Plasmid M17-2407  

A multi-gene quantification plasmid

Insert Gene names: MIG, MIP-ly, MlP-2, MIP-lα, TNF-α, ILI-β  IL-6, β-actin MCP2, RANTES, Eotaxin, Fractalkine, Lymphotactin, MIF, IP-I0, MIP-β, and HPRT

Insert size:                2500bp

Species:                   Murine

Vector backbone:    Mo-pCCy17

 

Plasmid M05-2407

A multi-gene quantification plasmid

Insert Gene names: CCL21, IFN-γ, IL-4, IL-10 and IL-12β

Species:                   Murine

Vector backbone:    mpCCy5

Useful for: Researchers working on gene expression, inflammation and inflammatory disease models.

Summary & Benefits:

  • Lowers the necessity to maintain multiple plasmid stocks and significantly minimises time and labour costs associated with RT-PCR.
  • Only one standard curve dilutional series has to be generated instead of 17 individual ones. Standard curves have been constructed for all 12 chemokines, 3 cytokines and the 2 housekeeping genes. Good linearity of the standard curves was noted for all of the chemokines and cytokines over the range 200 - 2 x 108 copies of target.
  • Sequence analysis confirms the orientation and authenticity of the cloned gene sequences.
  • All sequences show >98% identity with the DNA sequences deposited in the NCBI database.

 

 

 

 

Internal case number: 2407

T17 Multi-gene Murine Cytokine/Chemokine Plasmid

T17 Multi-gene Murine Cytokine/Chemokine Plasmid for Quantitative PCR

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2407 Plasmids 0.10

pre-licence

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Further details
Term: 12 months
Seats: n/a

Price excl. VAT: P.O.A. (price on application)

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