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Novel system enabling researchers to amplify and compare expression of multiple genes in a quicker and less resource-consuming way.

Setting up plasmid standard curves for quantitative RT-PCR can be time-consuming. This novel system allows researchers to amplify and compare expression of multiple genes in a quicker and less resource-consuming way. It reduces the variation between individual gene PCR amplification results due to plasmid dilutional errors. The invention reduces the necessity to maintain multiple plasmid stocks and significantly reduces time and labour costs associated with RT-PCR as only one serial dilution of plasmid is required for all the genes.

Reagents:

Plasmid HU14-2407

A multi-gene quantification plasmid

Insert Gene names: IL-2, MIF, Rantes, HPRT, IL-1β, IL-6, IFN-γ, TNF-α, MIP-2α, β-actin, MIP-1α, MIP-1β, MCP-1 and IL-8.

Insert size:                2400bp

Species:                   Human

Vector backbone:    Hu-pCCγ14

Plasmid HU03-2407

A multi-gene quantification plasmid

Insert Gene names: β-actin, HLA-DR, and IL-10

Species:                   Human

Vector backbone:    Hu-pCCγ3

Useful for: Researchers working on gene expression, inflammation and inflammatory disease models.

Summary & Benefits:

  • Lowers the necessity to maintain multiple plasmid stocks and significantly minimises time and labour costs associated with RT-PCR.
  • Only one standard curve dilutional series has to be generated instead of 14 individual ones. Standard curves have been constructed for all chemokines, cytokines and the housekeeping genes. Good linearity of the standard curves was noted for all of the chemokines and cytokines over the range 200 - 2 x 108 copies of target.
  • Sequence analysis confirms the orientation and authenticity of the cloned gene sequences.
  • All sequences show >98% identity with the DNA sequences deposited in the NCBI database.

Plasmid Map

 

Internal case number: 2527

T18 Multigene Human Cytokine/Chemokine Plasmids

T18 Multigene Human Cytokine/Chemokine Plasmids for Quantitative PCR

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2407 Plasmids 0.10

pre-licence

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Further details
Term: 12 months
Seats: n/a

Price excl. VAT: P.O.A. (price on application)

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