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Novel system enabling researchers to amplify and compare expression of multiple genes in a quicker and less resource-consuming way.

Setting up plasmid standard curves for quantitative RT-PCR can be time-consuming. This novel system allows researchers to amplify and compare expression of multiple genes in a quicker and less resource-consuming way. It reduces the variation between individual gene PCR amplification results due to plasmid dilutional errors. The invention reduces the necessity to maintain multiple plasmid stocks and significantly reduces time and labour costs associated with RT-PCR as only one serial dilution of plasmid is required for all the genes

Reagent:

 

Plasmid Rb05-2407

Multi-gene quantification plasmid

 

Insert Gene names: GAPDH, TNF-α, MIP-1β, IL-6, IL-8

Insert size:                700bp

Species:                   Rabbit

Vector backbone:    Ra-pCCy5

 

Plasmid Rb07-2407

Multi-gene quantification plasmid

 

Insert Gene names: IFN-β, IFN-γ, IL-12p35, IL-12p40 IL-1β, HPRT, IL-10

Insert size:                900bp

Species:                   Rabbit

Vector backbone:    Ra-pCCy7

 

Useful for: Researchers working on gene expression, inflammation and inflammatory disease models.

Summary & Benefits:

  • Lowers the necessity to maintain multiple plasmid stocks and significantly minimises time and labour costs associated with RT-PCR.
  • Only one standard curve dilutional series has to be generated instead of 12 individual ones.
  • Standard curves have been constructed for all 2 chemokines, 7 cytokines and the 2 housekeeping genes. Good linearity of the standard curves was noted for all of the chemokines and cytokines over the range 200 - 2 x 108 copies of target.
  • Sequence analysis confirms the orientation and authenticity of the cloned gene sequences. All sequences show >98% identity with the DNA sequences deposited in the NCBI database

Plasmid Maps

Plasmid Rb05-2407

 

Plasmid Rb07-2407

 

Internal case number: 7135

T19 Multigene Rabbit Cytokine/Chemokine Plasmids

T19 Multigene Rabbit Cytokine/Chemokine Plasmids for Quantitative PCR

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2407 Plasmids 0.10

pre-licence

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Further details
Term: 12 months
Seats: n/a

Price excl. VAT: P.O.A. (price on application)

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