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Chayen Reddy MIP is the first non-protein nucleating agent specifically designed for automated protein crystallization trials. This 'smart material' is a cost-effective tool for use in both screening and optimisation for scientists in academia and industry and has been tested on both the Mosquito (TTP) and Oryx (Douglas) protein crystallisation robots. Developed by Prof. Naomi Chayen at Imperial College London and Dr. Sub Reddy at the University of Surrey.

  • The first semi-liquid, non-protein nucleant compatible with automated crystallisation trials1.

  • Capable of nucleating a broader range of proteins than non-imprinted polymer substrate and other common nucleants, including the first ‘useful’ crystals of α-crustacyanin and intracellular xylanase IXT62.

  • Better quality crystals (higher resolution achieved) relative to non-imprinted polymer, including first resolution of human macrophage migration inhibitory factor (hMIF) without the need for synchrotron2.

 


hMIF crystal nucleated from Chayen-Reddy MIPs

 

 Product details:

  • MIPS have similar viscosity of corn oil and can be dispensed as an additive using any commercially available robot.

  • MIPS are available as a library containing 5 references at varying molecular weights from 14-500KDa:

    • 14KDa
    • 24KDa
    • 60KDa
    • 100KDa
    • >250KDa

  • MIPS are presented in vials containing 100 microlitres of the material, sufficient for 5000 experiments (approx.. 50 screens).

  • MIPS are to be stored at 4˚C and have a long shelf life (may require vortexing if unused for 3-4 weeks)

  • It is recommended to choose a MIP comparable to the molecular weight of your biomacromolecule for optimisation trials, but with approximately twice the molecular weight when screening. 

  • It is imperative to ensure the MIP added to the crystallisation droplet comprises no more than 10% of the trial volume.

 

 

Professor Naomi Chayen

Naomi E. Chayen is Professor of Biomedical Sciences at Imperial College London and the Head of the crystallization group in Computational and Systems Medicine. She specializes in the crystallization of proteins and other biological macromolecules, in particular, developing a fundamental understanding of the crystallization process and exploiting this to design practical methodology (including high-throughput methods) for producing high quality crystals of medical and industrial interest. Naomi has developed many unique methods adopted by crystal growth laboratories worldwide, several of which are commercialised (recently Naomi’s Nucleant; Chayen Reddy MIP).  Her methods have resulted in successful crystallization, leading to the structure determination of numerous proteins including membrane proteins and large macromolecular complexes that had previously failed to crystallize using conventional techniques.  She has received several awards among them an Innovator of the Year Prize and Women of Outstanding Achievement for Innovation and Entrepreneurshipc ommendation. 

Visit Naomi's page at Imperial College London

 

References:

1.       Khurshid et al. (2015) Automating the application of smart materials for protein crystallization, Acta Crystallographica D 71(3): 534-40

 

2.       Saridakis et al. (2011) Protein crystallization facilitated by molecularly imprinted polymers, Proceedings of the National Academy of Science USA 108(45): 18566 

 

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Chayen Reddy MIP

Nucleating agent compatible with protein crystallization robots

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