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Visualisation of de novo protein synthesis using click-chemistry in combination with simultaneous immunofluorescence. Using in vivo pulse-labelling with homopropargylglycine (HPG), an alkyne-modified analogue of methionine, followed by click-chemistry to couple bioorthogonal fluorochrome-incorporating capture reagents reveals the spatial distribution and relationship of newly synthesised proteins (white) during a 30 minutes pulse with viral proteins namely ICP0 (blue) and ICP22 (red). The ability to visualise protein synthesis in a spatially and temporally defined manner provides us a gateway to study metabolic responses in both uninfected and infected cells.

This image won the People's Choice Prize at the Imperial Innovations Art of Research competition.

The cells used were COS-1 (simian kidney) obtained from the European Collection of Authenticated Cell Cultures (ECACC), based at Public Health England.

 

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Commercialised on behalf of providing scientist. Internal case number: 7976

The Heart of Life

Visualisation of de novo protein synthesis using click-chemistry in combination with simultaneous immunofluorescence

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